[Bioperl-l] Bio::SeqFeature::Primer Calculating the Primer TM

Rob Edwards redwards at utmem.edu
Tue Feb 17 20:21:02 EST 2004


Thanks for this code. I really appreciate having an alternate method 
for calculating this. When I wrote the module I just couldn't figure 
out why the numbers wouldn't match. It seems Tm calculations are not as 
straightforward as they should (?) be. I always use 60C for my PCR 
annealing step and it works 99% of the time :-)

I added your code to Bio::SeqFeature::Primer in CVS and updated 
t/Primer.pm too so that it passes.

I left the original calculation in the module, but renamed it 
Tm_estimate so that it can be there for comparative purposes.


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