[Bioperl-l] Forward of moderated message
redwards at utmem.edu
Mon Mar 8 12:09:02 EST 2004
Are you sure that you are using the right module. This has been fixed
in Bio::Restriction (ver. 1.4)
my $renz=Bio::Restriction::Enzyme->new(-enzyme=>'MnII', -seq=>'^CCTC');
my $ranal=Bio::Restriction::Analysis->new(-seq=>$seq, -enzymes=>$renz);
print join "\n", @frags, "\n";
On Mar 8, 2004, at 10:43 AM, bioperl-guts-l-bounces at portal.open-bio.org
> From: Ruifang <zhangrf at xysm.net>
> Date: March 8, 2004 1:28:13 AM CST
> To: bioperl-guts-l at portal.open-bio.org
> Subject: a problem of RestrictionEnzyme
> I am a graduate student in National Lab of Medical Genetics of China.
> I am using Bioperl Module: RestrictionEnzyme.pm and I have encountered
> a question about this code.
> I found the restriction enzymes could not recognize the DAN sequence
> which is reverse complement to the enzymes sequence.
> For example, the enzyme: MnlI, the recognize sequence :CCTC, the DNA
> sequence: GTACTTTTAGAGGATGAAACCT
> the DAN sequence include GAGG , which is reverse complement to CCTC,
> and the MnlI should cut this sequence, but the code could not give
> this result.
> May I could have answer from you soon!
> Best Wishes!
> Bioperl-l mailing list
> Bioperl-l at portal.open-bio.org
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