[Bioperl-l] Ask help for Bio::Tools::Run::Primer3

Ewing, Adam D. EwingAD at hiram.edu
Wed Sep 29 16:38:18 EDT 2004

Yes, it does matter... simply change the name of primer3_core to primer3, put it in your path, and you should be good to go!


-----Original Message-----
From: bioperl-l-bounces at portal.open-bio.org on behalf of Fengkai Zhang (Tony)
Sent: Wed 9/29/2004 4:21 PM
To: bioperl-l at bioperl.org
Subject: [Bioperl-l] Ask help for Bio::Tools::Run::Primer3
Hi, I am a newbie for bioperl and met the below question.

I was trying to use Bio::Tools::Run::Primer3. When I run the example
code in the documentation of this module, I got the following error
<shell> $ perl -I ../primer3_1.0.0/src/ -w primer3run_test.pl 

Use of uninitialized value in concatenation (.) or string at
/usr/lib/perl5/site_perl/5.8.0/Bio/Tools/Run/Primer3.pm line 212,
<GEN0> line 1.
primer3 can not be found. Is it installed?

I already installed Primer3 according its instruction and it works
well to run the test and example. I also noticed that the
primer_test.pl in primer3 package calls "primer3_core" rather than
"primer3". Does it matter for Bio::Tools::Run::Primer3 module?

Thanks ahead for your help.


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