[Bioperl-l] Blast Connection Error

박준형 jhpark98 at dreamwiz.com
Tue Apr 5 22:59:55 EDT 2005


Until last two week, I could remote-blast against NCBI nr DB using bioperl
module. But right now, I can not remote-blast since a week.

I had set up interval time as 5 second. In my opinion, NCBI is not allowed
to blast search against NCBI nr DB. If you have experience like me, then
let me know.

Usually I do blast search for 50-100 sequences at one time.


The error message is as like this

-------------------- WARNING ---------------------

MSG: req was POST http://www.ncbi.nlm.nih.gov/blast/Blast.cgi

User-Agent: bioperl-Bio_Tools_Run_RemoteBlast/1.4

Content-Length: 336

Content-Type: application/x-www-form-urlencoded





<HEAD><TITLE>An Error Occurred</TITLE></HEAD>


<H1>An Error Occurred</H1>

500 Can't connect to www.ncbi.nlm.nih.gov:80 (Bad hostname



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