[Bioperl-l] Remote::Blast

CHANDAN SINGH chandan.kr.singh at gmail.com
Thu Apr 14 02:31:00 EDT 2005

Hi all 
I am facing the same problem and have alredy posted it on this forum .
In my case ,most of the time the error 500 is 
500 Can't connect to www.ncbi.nlm.nih.gov:80 (connect: No route to host)
sometimes i  also get the errror 500 as 
500 Can't connect to www.ncbi.nlm.nih.gov:80 (connect: timeout)

hello Thiago Motta Venancio.Are u using a proxy server to connect?
I somehow suspect that it has something to do with proxy.

Of late I have been trying to trace out the bug and found that 
The subroutine    UserAgent ::_need_proxy         returns an undef value 
in my case .
I would like to know more about this  subroutine and request all who
know about it to please respond .

On 4/14/05, Thiago Motta Venancio <venancio at ime.usp.br> wrote:
> Hi all.
> I am using the Remote::Blast module.
> The script was running ok, but it become out because of a 500 error and
> gaves a timeout www.ncbi.nih.go:80.
> Later, it came back, but the vast majority of sequences returned no
> matches, some of them are not really no matches.
> Any lights?
> Thanks in advance
> Thiago
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