[Bioperl-l] Entrez Gene parser questions
Annie.Law at nrc-cnrc.gc.ca
Thu Jun 9 14:57:40 EDT 2005
Thanks for everybody's responses. Yes, if I turn off the warnings then the scripts works. I was sticking with bioperl 1.4 because I think I read somewhere that the even extensions are the stable ones and Also I was happy with how 1.4 was working for me (and it seems that 1.4 works okay with the Entrez gene parser now) but if it is unadvised to plug in new modules to an older version then I will look into installing Bioperl 1.5. It seems to be fine for a lot of people :)
Just some questions about getting rid of bioperl 1.4. Am I correct in thinking that to 'uninstall' there is no such Uninstall command but I would just delete the /usr/lib/perl5/site_perl/5.8.0/Bio ie. The Bio directory and then Use CPAN to install bioperl 1.5
I am running my simple script and some print statements and it takes about 10 minutes to print 2000 Entrez gene Ids. How long does it generally take the parser to finish for example the Homo sapiens file? My real goal is not just to print but I just wanted to do a test run. Is this about the same performance others are getting or are there some other options to speed it up?
Thanks again for all of your efforts!
From: Stefan Kirov [mailto:skirov at utk.edu]
Sent: Wednesday, June 08, 2005 4:50 PM
To: Mingyi Liu
Cc: Law, Annie; Bioperl list
Subject: Re: [Bioperl-l] Entrez Gene parser questions
Sure. Thanks for letting me know.
Annie, does it work for you now?
Mingyi Liu wrote:
> Stefan's right in suggesting you turn off -w, which would make your
> script work. But thanks for finding this bug. I just noticed that
> entrezgene.pm was actually calling the Bio::ASN1::EntrezGene->next_seq
> incorrectly (probably my documentation was a bit confusing & my module
> did not follow standard hash-based parameter passing of subroutines).
> It should be called like ->next_seq(1), but entrezgene.pm called using
> ->next_seq(-trimopt => 1). This worked for all of us who do not use
> -w, as it would fall back to option '1' in my next_seq function
> (exactly as Stefan's calling function wanted). Therefore this bug
> went unnoticed until you turned on -w (I guess we were all spoiled by
> the easy (and sometimes messy) life of weak datatyping in Perl).
> Stefan would you please change the calling of next_seq to next_seq(1)?
> This would fix the error messages Annie was seeing.
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