[Bioperl-l] More fun with primer3
muratem at eng.uah.edu
Tue Mar 29 12:13:22 EST 2005
Having deduced (and heard from Rob) that Tools::Run::Primer3 produces a
Tools::Primer3 object, I tried the following:
my $results = $primer3->run;
print "n results ",$results->number_of_results(),"\n";
my $primer = $results->next_primer();
n results 4
------------- EXCEPTION -------------
MSG: The target_sequence must be a Bio::Seq to create this object.
STACK toplevel ./extractAlignments.pl:209
I'm at a loss, it's pretty much a cut and paste out of the docs.
Does anybody have any ideas?
PS The perldoc (I have) of Bio::Tools::Run::Primer3 says it returns a
Bio::Tools::Run::Primer3 object and not Bio::Tools::Primer3. I think it's
the latest version.
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