[Bioperl-l] newbie tries StandAloneBlast and receives "cant open BLOSUM62"
torsten.seemann at infotech.monash.edu.au
Thu Mar 2 05:50:44 EST 2006
> I am a Bioinformatics Newbie and want to use BioPerl for doing BLASTs on
> a local protein-sequence file. Unfortunately something went wrong, which
> gives me the error message: "...Unable to open BLOSUM62...".
> [NULL_Caption] WARNING: [000.000] test_query: Unable to open BLOSUM62
> [NULL_Caption] WARNING: [000.000] test_query: BlastScoreBlkMatFill
> returned no
> n-zero status
> [NULL_Caption] WARNING: [000.000] test_query: SetUpBlastSearch failed.
> Does anyone has a clue, what is going wrong? Since my ncbi.ini - file
> points to the directory, where blosum62 is found, I can not understand
> this error-message.
It really looks like it can't find BLOSUM62, which indicates that the
ncbi.ini file is not being read, or has the wrong syntax.
Those "/" forward slashes look potentially problematic on a Windows
platform - perhaps try using "\" backslashes. ie.
Just an idea,
--Victorian Bioinformatics Consortium, Australia.
More information about the Bioperl-l