[Bioperl-l] newbie tries StandAloneBlast and receives "cant open BLOSUM62"
cjfields at uiuc.edu
Thu Mar 2 09:08:24 EST 2006
Torsten's right. This is what I have for BLASTDIR:
C:\Documents and Settings\Administrator>echo %BLASTDIR%
Postdoctoral Researcher - Switzer Lab
Dept. of Biochemistry
University of Illinois Urbana-Champaign
> -----Original Message-----
> From: bioperl-l-bounces at lists.open-bio.org [mailto:bioperl-l-
> bounces at lists.open-bio.org] On Behalf Of Torsten Seemann
> Sent: Thursday, March 02, 2006 4:51 AM
> To: bioperl-l at lists.open-bio.org
> Cc: Harald
> Subject: Re: [Bioperl-l] newbie tries StandAloneBlast and receives "cant
> open BLOSUM62"
> > I am a Bioinformatics Newbie and want to use BioPerl for doing BLASTs on
> > a local protein-sequence file. Unfortunately something went wrong, which
> > gives me the error message: "...Unable to open BLOSUM62...".
> > ==============C:/WINNT/ncbi.ini========================
> > [NCBI]
> > Data="D:/BLAST/BLAST-2.2.13/data"
> > ===================================================
> > [NULL_Caption] WARNING: [000.000] test_query: Unable to open BLOSUM62
> > [NULL_Caption] WARNING: [000.000] test_query: BlastScoreBlkMatFill
> > returned no
> > n-zero status
> > [NULL_Caption] WARNING: [000.000] test_query: SetUpBlastSearch failed.
> > 0
> > Does anyone has a clue, what is going wrong? Since my ncbi.ini - file
> > points to the directory, where blosum62 is found, I can not understand
> > this error-message.
> It really looks like it can't find BLOSUM62, which indicates that the
> ncbi.ini file is not being read, or has the wrong syntax.
> Those "/" forward slashes look potentially problematic on a Windows
> platform - perhaps try using "\" backslashes. ie.
> Just an idea,
> --Torsten Seemann
> --Victorian Bioinformatics Consortium, Australia.
> Bioperl-l mailing list
> Bioperl-l at lists.open-bio.org
More information about the Bioperl-l