[Bioperl-l] Memory Leak in Bio::SearchIO

Clarke, Wayne ClarkeW at AGR.GC.CA
Mon May 15 15:40:15 EDT 2006

Hey everyone, 


I have been developing some code to download and parse blast reports
from a remote server using Soap::Lite as well as insert the results into
a mysql database. The problem I am having is that my program seems to be
taking up and huge amount of RAM. For a single job of 10000 queries it
can consume as much as a couple hundred Mb inside an hour. I realize
that a lot of work is being done but this seems like way too much. This
leads me to the subject of my post. I think I may have traced the source
of the memory leak to Bio::SearchIO. I have used Devel::Size to track
the size of my variables and done other debugging steps and have had no
luck with resolving this very frustrating problem. My code is as


 my $result = $connector->getQueryResult($query_id);


                my $FH;

                open $FH, "<", \$result;


                my $searchio = new Bio::SearchIO(-format => "blast",


                         -fh => $FH);


                while (my $o_blast = $searchio->next_result()) {

                        my $clone_id = $o_blast->query_name();


                        my $statement = $bdbi->form_push_SQL ($o_blast,
$clone_id, 5);


this is just the leading and tailing code surrounding the use of
Bio::SearchIO since there is quite a lot. I am mostly just wondering if
anyone has ever had problems with SearchIO and its memory usage. I
looked at the source code for it but am afraid it is out of my league.
Any help/suggestions/questions would be great. Thanks

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