[Bioperl-l] help on remote blast

Sanjib Kumar Gupta sanjib at bic.boseinst.ernet.in
Wed Feb 21 01:12:06 EST 2007


Hi
I have been running this script for some time and it was running fine. I am 
using this linux machine with live IP(no proxy). But suudenly it has stopped 
working with this errors


waiting...waiting...
-------------------- WARNING ---------------------
MSG: <HTML>
<HEAD><TITLE>An Error Occurred</TITLE></HEAD>
<BODY>
<H1>An Error Occurred</H1>
500 Can't connect to www.ncbi.nlm.nih.gov:80 (Bad 
hostname 'www.ncbi.nlm.nih.gov')
</BODY>
</HTML>
 
---------------------------------------------------
xx.pep
 
-------------------- WARNING ---------------------
MSG: req was POST http://www.ncbi.nlm.nih.gov/blast/Blast.cgi
User-Agent: bioperl-Bio_Tools_Run_RemoteBlast/1.5
Content-Length: 497
Content-Type: application/x-www-form-urlencoded
 
DATABASE=nr&QUERY=%3ENM_005277.3%3B_1414961_1+837%
0AMEENMEEGQTQKGCFECCIKCLGGIPYASLIATILLYAGVALFCGCGHEALSGTVNILQTYFEMARTAGDTLDVF
TMIDIFKYVIYGIAAAFFVYGILLMVEGFFTTGAIKDLYGDFKITTCGRCVSAWFIMLTYLFMLAWLGVTAFTSLPV
YMYFNLWTICRNTTLVEGANLCLDLRQFGIVTIGEEKKICTVSENFLRMCESTELNMTFHLFIVALAGAGAAVIAMV
HYLMVLSANWAYVKDACRMQKYEDIKSKEEQELHDIHSTRSKERLNAYT*&COMPOSITION_BASED_STATISTI
CS=off&EXPECT=1e-
10&SERVICE=plain&FORMAT_OBJECT=Alignment&CMD=Put&MATRIX_NAME=BLOSUM62&ENTREZ_
QUERY=Xenopus+laevis[Organism]&PROGRAM=blastp
 
<HTML>
<HEAD><TITLE>An Error Occurred</TITLE></HEAD>
<BODY>
<H1>An Error Occurred</H1>
500 Can't connect to www.ncbi.nlm.nih.gov:80 (Bad 
hostname 'www.ncbi.nlm.nih.gov')
</BODY>
</HTML>
 
---------------------------------------------------
waiting...waiting...
-------------------- WARNING ---------------------
MSG: <HTML>
<HEAD><TITLE>An Error Occurred</TITLE></HEAD>
<BODY>
<H1>An Error Occurred</H1>
500 Internal Server Error
</BODY>
</HTML>
 
---------------------------------------------------

Though I am able to see the ncbi page from browser but am unable to ping ot 
trace route to the server.

Please help me.
--
Sanjib Kumar Gupta
Bioinformatics Centre
Bose Institute
Kolkata 700054, INDIA
Phone  : +91-33-2355 6626, 2816, 2355 4766
Fax    : +91-33-2355 3886
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