[Bioperl-l] Taxonomy hierarchy extraction

George Heller george.heller at yahoo.com
Mon Jun 18 20:04:00 EDT 2007


Ok, I installed the latest of Scalar::Util and the script seems to be working. But I am confused where exactly I need to look for the descendent taxon ids once the script is run. I did look into the /tmp/ directory, but I couldnt understand much. 
   
  Sorry to be bothering, really appreaciate your patience.
   
  Thanks.
  George

Jason Stajich <jason at bioperl.org> wrote:
  Try installing the latest Scalar::Util  
    On Jun 18, 2007, at 4:05 PM, George Heller wrote:

    This is the output of /usr/bin/perl -V
  

  Summary of my perl5 (revision 5 version 8 subversion 5) configuration:
    Platform:
      osname=linux, osvers=2.6.9-22.18.bz155725.elsmp, archname=i386-linux-thread-multi
      uname='linux hs20-bc1-4.build.redhat.com 2.6.9-22.18.bz155725.elsmp #1 smp thu nov 17 15:34:08 est 2005 i686 i686 i386 gnulinux '
      config_args='-des -Doptimize=-O2 -g -pipe -m32 -march=i386 -mtune=pentium4 -Dversion=5.8.5 -Dmyhostname=localhost -Dperladmin=root at localhost -Dcc=gcc -Dcf_by=Red Hat, Inc. -Dinstallprefix=/usr -Dprefix=/usr -Darchname=i386-linux -Dvendorprefix=/usr -Dsiteprefix=/usr -Duseshrplib -Dusethreads -Duseithreads -Duselargefiles -Dd_dosuid -Dd_semctl_semun -Di_db -Ui_ndbm -Di_gdbm -Di_shadow -Di_syslog -Dman3ext=3pm -Duseperlio -Dinstallusrbinperl -Ubincompat5005 -Uversiononly -Dpager=/usr/bin/less -isr -Dinc_version_list=5.8.4 5.8.3 5.8.2 5.8.1 5.8.0'
      hint=recommended, useposix=true, d_sigaction=define
      usethreads=define use5005threads=undef useithreads=define usemultiplicity=define
      useperlio=define d_sfio=undef uselargefiles=define usesocks=undef
      use64bitint=undef use64bitall=undef uselongdouble=undef
      usemymalloc=n, bincompat5005=undef
    Compiler:
      cc='gcc', ccflags ='-D_REENTRANT -D_GNU_SOURCE -DDEBUGGING -fno-strict-aliasing -pipe -I/usr/local/include -D_LARGEFILE_SOURCE -D_FILE_OFFSET_BITS=64 -I/usr/include/gdbm',
      optimize='-O2 -g -pipe -m32 -march=i386 -mtune=pentium4',
      cppflags='-D_REENTRANT -D_GNU_SOURCE -DDEBUGGING -fno-strict-aliasing -pipe -I/usr/local/include -I/usr/include/gdbm'
      ccversion='', gccversion='3.4.6 20060404 (Red Hat 3.4.6-2)', gccosandvers=''
      intsize=4, longsize=4, ptrsize=4, doublesize=8, byteorder=1234
      d_longlong=define, longlongsize=8, d_longdbl=define, longdblsize=12
      ivtype='long', ivsize=4, nvtype='double', nvsize=8, Off_t='off_t', lseeksize=8
      alignbytes=4, prototype=define
    Linker and Libraries:
      ld='gcc', ldflags =' -L/usr/local/lib'
      libpth=/usr/local/lib /lib /usr/lib
      libs=-lresolv -lnsl -lgdbm -ldb -ldl -lm -lcrypt -lutil -lpthread -lc
      perllibs=-lresolv -lnsl -ldl -lm -lcrypt -lutil -lpthread -lc
      libc=/lib/libc-2.3.4.so, so=so, useshrplib=true, libperl=libperl.so
      gnulibc_version='2.3.4'
    Dynamic Linking:
      dlsrc=dl_dlopen.xs, dlext=so, d_dlsymun=undef, ccdlflags='-Wl,-E -Wl,-rpath,/usr/lib/perl5/5.8.5/i386-linux-thread-multi/CORE'
      cccdlflags='-fPIC', lddlflags='-shared -L/usr/local/lib'
  

  Characteristics of this binary (from libperl):
    Compile-time options: DEBUGGING MULTIPLICITY USE_ITHREADS USE_LARGE_FILES PERL_IMPLICIT_CONTEXT
    Built under linux
    Compiled at Jul 24 2006 18:28:10
    @INC:
      /usr/lib/perl5/5.8.5/i386-linux-thread-multi
      /usr/lib/perl5/5.8.5
      /usr/lib/perl5/site_perl/5.8.5/i386-linux-thread-multi
      /usr/lib/perl5/site_perl/5.8.4/i386-linux-thread-multi
      /usr/lib/perl5/site_perl/5.8.3/i386-linux-thread-multi
      /usr/lib/perl5/site_perl/5.8.2/i386-linux-thread-multi
      /usr/lib/perl5/site_perl/5.8.1/i386-linux-thread-multi
      /usr/lib/perl5/site_perl/5.8.0/i386-linux-thread-multi
      /usr/lib/perl5/site_perl/5.8.5
      /usr/lib/perl5/site_perl/5.8.4
      /usr/lib/perl5/site_perl/5.8.3
      /usr/lib/perl5/site_perl/5.8.2
      /usr/lib/perl5/site_perl/5.8.1
      /usr/lib/perl5/site_perl/5.8.0
      /usr/lib/perl5/site_perl
      /usr/lib/perl5/vendor_perl/5.8.5/i386-linux-thread-multi
      /usr/lib/perl5/vendor_perl/5.8.4/i386-linux-thread-multi
      /usr/lib/perl5/vendor_perl/5.8.3/i386-linux-thread-multi
      /usr/lib/perl5/vendor_perl/5.8.2/i386-linux-thread-multi
      /usr/lib/perl5/vendor_perl/5.8.1/i386-linux-thread-multi
      /usr/lib/perl5/vendor_perl/5.8.0/i386-linux-thread-multi
      /usr/lib/perl5/vendor_perl/5.8.5
      /usr/lib/perl5/vendor_perl/5.8.4
      /usr/lib/perl5/vendor_perl/5.8.3
      /usr/lib/perl5/vendor_perl/5.8.2
      /usr/lib/perl5/vendor_perl/5.8.1
      /usr/lib/perl5/vendor_perl/5.8.0
      /usr/lib/perl5/vendor_perl
  

    Thanks.
    George
      .
  

  Hilmar Lapp <hlapp at gmx.net> wrote:
    The perl version appears to be 5.8.5 though, so something strange 
  appears to be going on too.
  

  George, can you please post the output of
  

  $ /usr/bin/perl -V
  

  -hilmar
  

  On Jun 18, 2007, at 6:33 PM, Chris Fields wrote:
  

    As the error implies your local version of perl doesn't seem support
  weak references, which means it doesn't have Scalar::Utils (which was
  added to core after perl 5.6.1, I think). Try installing
  Scalar::Utils to see what happens.
  

  chris
  

  On Jun 18, 2007, at 5:18 PM, George Heller wrote:
  

    I tried running the below mentioned script and I seem to be getting
  the following error:
  

  Weak references are not implemented in the version of perl at /
  usr/lib/perl5/site_perl/5.8.5/Bio/Tree/Node.pm line 76
  BEGIN failed--compilation aborted at /usr/lib/perl5/site_perl/5.8.5/
  Bio/Tree/Node.pm line 76.
  Compilation failed in require at my.pl line 7.
  BEGIN failed--compilation aborted at my.pl line 7.
  

  My script looks something like,
  

  #!/usr/bin/perl
  use strict;
  #use warnings;
  use DBI;
  use Bio::Tree::Node;
  use Bio::DB::Taxonomy;
  use Bio::DB::Taxonomy::flatfile;
  my $idx_dir = '/tmp';
  

  my ($nodefile,$namesfile) = ('nodes.dmp','names.dmp');
  my $db = new Bio::DB::Taxonomy(-source => 'flatfile',
  -nodesfile => $nodesfile,
  -namesfile => $namesfile,
  -directory => $idx_dir);
  my $node = $db->get_Taxonomy_Node(-taxonid => '33090');
  my @extant_children = grep { $_->is_Leaf } $node-
    get_all_Descendents;
  

  foreach $field (@extant_children) {
  print "$field";
  print "|";
  print "\n";
  }
  

  And I am running the script using the command,
  

  perl myscript.pl -v --names names.dmp --nodes nodes.dmp
  

  and I have the nodes.dmp and names.dmp files in the current
  directory.
  

  Thanks,
  George
  

  

  Jason Stajich wrote:
  It is implemented in the implementing class - DB::Taxonomy is
  just the base class. For example see the flatfile implementation
  Bio::DB::Taxonomy::flatfile
  

  See the scripts/taxa/local_taxonomydb_query.PLS for example using
  it:
  nodes and names are from NCBI taxonomy database.
  

  

  Here is an un-debugged copy+paste for your question that *should*
  work.
  

  

  use Bio::DB::Taxonomy
  my $idx_dir = '/tmp';
  

  

  my ($nodefile,$namesfile) = ('nodes.dmp,'names.dmp');
  my $db = new Bio::DB::Taxonomy(-source => 'flatfile',
  -nodesfile => $nodesfile,
  -namesfile => $namesfile,
  -directory => $idx_dir);
  my $node = $db->get_Taxonomy_Node(-taxonid => '33090');
  my @extant_children = grep { $_->is_Leaf } $node-
    get_all_Descendents;
  

  

  

  

  -jason
  

  On Jun 18, 2007, at 10:07 AM, George Heller wrote:
  

  What exactly is the "node n" in the query below. When I issue
  this query, it says,
  

  

  relation "node" does not exist.
  

  

  I tried to use the get_all_Descendents method but it looks like
  in order to do a recursive call it calls the method
  each_Descendent. This method is not implemented in
  Bio::DB::Taxonomy. It just has a single line,
  

  

  shift->throw_not_implemented();
  

  

  Thanks.
  George.
  

  

  Hilmar Lapp wrote:
  I'm a bit confused - it sounds like you have set up a local 
  BioSQL
  database and loaded the NCBI taxonomy into the database. You can 
  now
  use simple SQL to retrieve all descendants of a node in the tree
  given its NCBI taxonID such as
  

  

  SELECT tn.*, tnm.name FROM taxon tn, taxon_name tnm, node n
  WHERE
  n.ncbi_taxon_id = :taxonID
  AND tn.left_value > n. left_value
  AND tn.right_value < n.right_value
  AND tn.taxon_id = tnm.taxon_id
  AND tn.name_class = 'scientific_name'
  

  

  BioPerl doesn't have a Taxonomy::biosql module yet (though this
  would
  seem like a worthwhile thing to add), so you can't use the
  Bio::DB::Taxonomy interface to do this against a BioSQL instance.
  

  

  However, BioPerl does have support for the flat-file download of 
  the
  NCBI taxonomy database and indexes it, so you can simply use
  Taxonomy::{get_taxon,get_all_Descendants} using the flatfile
  download
  to achieve what you wanted to do in a less than 5 lines of perl.
  

  

  Although the recursive implementation of
  Taxonomy::get_all_Descendants
  () won't be lightning fast, it may still be perfectly fine for your
  application - are you sure it is not?
  

  

  -hilmar
  

  

  On Jun 18, 2007, at 12:21 AM, George Heller wrote:
  

  

  Thanks. And how can I assign the $node here in the below code,
  such
  that I can reference it to a particular taxon id record? I want to
  retrieve all the descendents from the taxonomy hierarchy, given a
  particular taxon id.
  

  

  I have a local db setup, in which I have uploaded data using the
  load_ncbi_taxonomy.pl script.
  

  

  Thanks.
  George
  

  

  Jason Stajich wrote:
  I assume you already figured out how to setup a local taxonomydb?
  

  

  

  

  You just want the extant species/leaves of the tree
  

  

  

  

  my @extant_children = grep { $_->is_Leaf } $node-
    get_all_Descedents;
  

  

  

  

  

  

  -jason
  On Jun 17, 2007, at 11:41 AM, George Heller wrote:
  

  

  Hi all,
  

  

  

  

  Can anyone point me to some example that uses the
  get_all_Descendents method from Bio::DB::Taxonomy? I am a newbie at
  this, and I am not quite sure how to implement it.
  

  

  

  

  Thanks.
  George
  

  

  

  

  Sendu Bala wrote:
  George Heller wrote:
  Hi all,
  

  

  

  

  I am looking at extracting the taxonomy hierarchy for some taxon
  ids.
  What I plan to do is, for a given taxon id, say 33090, I want to
  extract all taxon ids that are children of this species. I do not
  just want the immediate children, but the children's children 
  and so
  on.
  

  

  

  

  Any ideas on the way I can go about doing this?
  

  

  

  

  Well, you'll use Bio::DB::Taxonomy presumably, and
  each_Descendent in
  some kind of looping structure. Most easily a recursing sub.
  

  

  

  

  If you happen to code up something neat and efficient, why not
  share it
  with us and we could add it to the Taxonomy module(s).
  

  

  

  

  

  

  

  

  

  

  

  

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  Lab of Dr. Robert Switzer
  Dept of Biochemistry
  University of Illinois Urbana-Champaign
  

  

  

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