[Bioperl-l] run RNAfold in perl

Chris Fields cjfields at uiuc.edu
Fri Nov 23 17:49:43 EST 2007

The Pise wrappers run the programs remotely; see  
Bio::Tools::Run::AnalysisFactory::Pise on how to run it.  As for a  
local RNAfold wrapper, I had planned on making Bioperl-based Vienna/ 
mfold wrappers but haven't done so yet.  The Vienna tools do have a  
Perl-based (non-BioPerl-based) module included which uses libRNA, and  
is well worth a look.  Try 'perldoc RNA' if you have installed the  
tools locally, or look here for other Perl-based tools:



On Nov 23, 2007, at 3:26 PM, vanam wrote:

> how to run RNAfold using Bio::Tools::Run::AnalysisFactory::Pise?????
> my $factory = Bio::Tools::Run::AnalysisFactory::Pise->new();
> my $rnafold = $factory->program('rnafold');
> my $job=$rnafold->run(-rnafold =>
> I installed Vienna package and then i tried using Pise to create an  
> object
> for the program but its giving the following error
> ------------- EXCEPTION: Bio::Root::Exception -------------
> MSG: Bio::Tools::Run::PiseJob terminated: URL missing
> STACK: Error::throw
> STACK: Bio::Root::Root::throw
> /usr/local/share/perl/5.8.8/Bio/Root/Root.pm:359
> STACK: Bio::Tools::Run::PiseJob::terminated
> /usr/local/share/perl/5.8.8/Bio/Tools/Run/PiseJob.pm:460
> STACK: Bio::Tools::Run::PiseApplication::submit
> /usr/local/share/perl/5.8.8/Bio/Tools/Run/PiseApplication.pm:416
> STACK: Bio::Tools::Run::PiseApplication::run
> /usr/local/share/perl/5.8.8/Bio/Tools/Run/PiseApplication.pm:352
> STACK: evaluate.pl:12
> how to make the program RNAfold run in perl...
> IS THERE ANY NEED TO SPECIFY WHERE MY rnafold program is???
> plz reply soon
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Christopher Fields
Postdoctoral Researcher
Lab of Dr. Robert Switzer
Dept of Biochemistry
University of Illinois Urbana-Champaign

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