[Bioperl-l] Creating a fastq format file?

Hotz, Hans-Rudolf hans-rudolf.hotz at fmi.ch
Wed Apr 22 10:58:21 EDT 2009

> Howdy
> This might be a good place to ask the question: having looked at the
> fastq.pm page, is the fastq format defined (only) by a "@'" followed
> by a sequence line and a "+" header followed by a quality line and the
> two headers have to agree? Now that Illumina is using phred scaling,
> are 'Sanger' and 'Illumina' versions the same?

No, see:

Regards, Hans

> Thanks
> Mike

More information about the Bioperl-l mailing list