[Bioperl-l] Tm calculation using thermodynamic concept for Degenerate Primers?
bhakti.dwivedi at gmail.com
Wed Apr 14 13:20:08 EDT 2010
Does anyone know how to compute the melting temperature for Degenerate
Primers following the thermodynamic nearest neighbor model? or how it
works? what I found so far is Tm calculations for primers with A,C,G, or T
only, but haven't came across anything that can help me explain the way it
works for degenerate primers, so I can implement that in my perl code.
any help please? Thanks!
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