[Bioperl-l] seq object usage

Roy Chaudhuri roy.chaudhuri at gmail.com
Tue Jun 12 09:54:55 EDT 2012

Bio::Seq's seq method just delegates to Bio::PrimarySeq:

So this should work:

You're right about the memory thing though. Math, couldn't you just 
print out a Fasta file directly rather than using BioPerl?


On 12/06/2012 14:28, Frank Schwach wrote:
> the Bio::Seq's "seq" method doesn't take an argument to set a new
> sequence, so I think you would have to do it like this:
> $bio_seq_obj->primary_seq(
> Bio:PrimarySeq->new(
> -seq => $bio_seq_obj->seq .$some_addition_seq
> )
> );
> but it wouldn't save you any memory because you still have the whole
> sequence in memory.
> On 12/06/12 11:27, math math wrote:
>> Hi,
>> consider the following case:
>> A seq object's string field (ie the sequence part) needs to be filled
>> gradually in a PERL loop. The seq object(s) can be hundreds of millions
>> bases long at the end of this loop. The final goal is to write this seq
>> object(s) to an output file (in multi-fasta format).
>> My question is, how can one append progressively more sequence to a seq
>> object (i.e. instead of all at once, to minimize memory), and make sure
>> that these get written to a file.
>> Thanks.
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>> Bioperl-l at lists.open-bio.org
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